The Research

Essam Abd Al-Sattar – Pharmacology (natural product), Ahmad Shaker Medical Pharmacology, Sawsan Rahim Aldin – Biochemistry, Jehan Fouad Medical Physics, Suad Shaker – Medical Biology, Hoda Abo Arki Veterinary Medicine, Wadeah Baker – Biochemistry, Najwa Al Sawi Biochemistry, Amin Al Jefri – Medicine, Sabah Mushrif – Medicine, Najwa Tawfiq – Tissue Culture. 

Abdel Moneim Osman-Medical Pharmacy, Awatif Mohammad – Medical biology, Hanan Al Shathli- Medicine , Manal Khorshid- Medicine , Nadiah Spki – Medicine, Zuhoor Al Gheithi – Medicine, Sami Alnuaim – Medicine, Alaa Keder - Pharmacology, Mahmoud Shahin- Oncology, Abdulwahab Badr - Dermatology, Samiha Al Shathli- Dermatology, Fadwa Khorshid- Medicine,Haifaa Janna-Dermatology, Dr.Hayfaa Janna-Medicine.. and others.

 Research Summery

Camel urine or PM 701 was clean, sterile and free from toxicity when it was studied by our team work. We used PM 701 as anticancer agent. The work was conducted as in vitro, in vivo, preclinical and clinical studies.

The study used the technique of tissue culture of human lung cancer cells and mice leukemia cells, which were compared to with normal human skin fibroblasts in studying the effect of PM 701. This proved that our agent could induce apoptosis of the cancer cells without affecting the normal cells. The in vivo studies tested the effect of PM 701 in treating MFI mice, which were inoculated with L1210 cells. The result of in vivo is satisfactory as in vitro, where the life span and the survival of animals improved. The histopathology studies revealed disappearance of malignant tumors in the organs of treated animals or at least a decrease the in the degree of metastasis. So PM 701 showed promising results as anticancer agent in cancer induced animal model. For enhancing the utility and convenience of application of PM701, liquid PM701 was lyophilized to reach a solid form. Then the solid form was fractionated to obtain the bioactive fraction(s), which were more readily acceptable to humans. During these steps, we monitored the activity of the different fractions using the different tests subjected previously on the crude PM 701, such as in vitro and in vivo tests. The fraction, which was coded PMF (150mg/g of the lyophilized PM701) was able to significantly inhibit the proliferation of cancer cells without affecting the normal cells at cell culture levels. So PMF is the ideal selective cytotoxic agent whereas the other fractions are less effective or non-selective. On further fractionation, PMF was subfractionated to seven parts in order to isolate the most effective cytotoxic constituents of this bio-active fraction. Our studies identified that the subfraction PMFK was the most cytotoxic constituents. PMF and PMFK had been examined and showed successful results in preventing the growth of cultivated cancer cells for different carcinogenic cell lines as in the liver, colon, brain and breast in addition to the lung and leukemia. The pharmacological and toxicological studies were done on formulated PM701 and PMF on animal models. These experiments proved their safety as indicated by the high value of MTD on animal models and also by its safety effect on vital organs of animal models through histopathological studies carried out using light and electron microcopies.